STUDY ON APPLICATION OF THIN CELL LAYER CULTURE FOR IN VITRO PROPAGATION OF CHRYSANTHEMUM INDICUM

Nguyen Van Viet

STUDY ON APPLICATION OF THIN CELL LAYER CULTURE FOR IN VITRO PROPAGATION OF CHRYSANTHEMUM INDICUM

Authors

Nguyen Van Viet Vietnam National University of Forestry

Keywords:

Callus, Chrysanthemum indicum, in vitro, propagation, thin cell layer

Abstract

Chrysanthemum indicum L. is a common flower which can bring highly economic, C. indicum is on of the most important cut flower and pot plant. Thin cell layer (TCL) culture is a potential method for in vitro propagation of C. indicum. However, this method is still limited in Vietnam. After sterilization with HgCl₂ 0.1% solution for 6 minutes and being cultured on Murashige T. and Skoog F. (1962) (MS) medium supplemented with 6-benzyl amino purine (BAP) 0.5 mg/l, α-naphthaleneacetic acid (NAA) 0.2 mg/l, sucrose 30 g/l, agar 7 g/l, the cultured samples were recorded with survival percentage of 81%, shoots were generated after 4 weeks. Callus induction and shoot regeneration on MS medium supplemented with BAP 0.5 mg/l, kinetin 0.2 mg/l, NAA 0.2 mg/l were obtained with 82.2% and 80%, respectively.

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Published

05-01-2024

How to Cite

Van Viet, N. (2024). STUDY ON APPLICATION OF THIN CELL LAYER CULTURE FOR IN VITRO PROPAGATION OF CHRYSANTHEMUM INDICUM. Journal of Forestry Science and Technology, (5), 037–042. Retrieved from https://jvnuf.vjst.net/en/article/view/1158